Search results for "DNA detection"

showing 7 items of 7 documents

2020

Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a s…

0303 health sciencesChromatographyPcr cloningPcr assay02 engineering and technologyDna recoveryLiquid nitrogen021001 nanoscience & nanotechnologyGeneral Biochemistry Genetics and Molecular BiologyDna detection03 medical and health scienceschemistry.chemical_compoundchemistryDigital polymerase chain reactionDroplet microfluidics0210 nano-technologyDNA030304 developmental biologyBiotechnologyBioTechniques
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Plasmonic Nanosensor Array for Multiplexed DNA-based Pathogen Detection

2019

In this research we introduce a plasmonic nanoparticle based optical biosensor for monitoring of molecular binding events. The sensor utilizes spotted gold nanoparticle arrays as sensing platform. The nanoparticle spots are functionalized with capture DNA sequences complementary to the analyte (target) DNA. Upon incubation with the target sequence, it will bind on the respectively complementary functionalized particle spot. This binding changes the local refractive index, which is detected spectroscopically as the resulting changes of the localized surface plasmon resonance (LSPR) peak wavelength. In order to increase the signal, a small gold nanoparticle label is introduced. The binding ca…

AnalyteMaterials scienceta221Molecular bindingMetal NanoparticlesNanoparticleBioengineering02 engineering and technologybiosensorit01 natural sciencesSensor arrayLimit of DetectionNanosensorplasmonic arrayNanotechnologyLSPR sensingSurface plasmon resonanceDNA FungalInstrumentationPlasmonCandidaDNA detectionFluid Flow and Transfer ProcessesPlasmonic nanoparticlesBase Sequenceta114business.industryProcess Chemistry and Technology010401 analytical chemistryta1182Fourier-transform-imaging spectroscopynanobiotekniikkaDNASurface Plasmon Resonance021001 nanoscience & nanotechnologyplasmonic nanoparticles0104 chemical sciencesAspergillusOptoelectronicsnanohiukkasetGoldDNA Probes0210 nano-technologybusinessACS Sensors
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Plasmonic nanosensor array for multiplexed DNA-based pathogen detection

2019

In this research we introduce a plasmonic nanoparticle based optical biosensor for monitoring of molecular binding events. The sensor utilizes spotted gold nanoparticle arrays as sensing platform. The nanoparticle spots are functionalized with capture DNA sequences complementary to the analyte (target) DNA. Upon incubation with the target sequence, it will bind on the respectively complementary functionalized particle spot. This binding changes the local refractive index, which is detected spectroscopically as the resulting changes of the localized surface plasmon resonance (LSPR) peak wavelength. In order to increase the signal, a small gold nanoparticle label is introduced. The binding ca…

DNA detectionplasmonic arrayFourier-transform-imaging spectroscopyLSPR sensingnanobiotekniikkananohiukkasetDNAbiosensoritplasmonic nanoparticles
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Penile, Uretral and Seminal Sampling for Diagnosis of Human Papillomavirus Infection in Men.

2006

ABSTRACT Methods that used specimens from three genital sites (penile brushing [PB], urethral brushing [UB], and the retrieval of semen [SE]) from 50 men were examined for human papillomavirus (HPV) DNA detection. The rates of detection by PB, UB, SE, PB and UB, and PB and SE were 88.9%, 50.0%, 33.3%, 100%, and 97.2%, respectively. The use of PB and UB appears to be the most accurate method; as an alternative to UB, the use of SE with PB could be used to improve the rate of HPV DNA detection in men.

MaleMicrobiology (medical)medicine.medical_specialtySemenPolymerase Chain ReactionSpecimen HandlingUrethraSemenVirologymedicinehuman papillomavirus HPVHumansSex organPapillomaviridaeHuman papillomavirusPapillomaviridaeGynecologybiologyPapillomavirus Infectionsbiology.organism_classificationDna detectionHPV DNA detectionmedicine.anatomical_structureUrethraDNA ViralWartsPenisPenis
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Orthokeratinized odontogenic cysts: a Spanish tertiary care centre study based on HPV DNA detection

2018

Abstract Background The role of human papillomavirus (HPV) in orthokeratinized odontogenic cysts (OOCs) has rarely been studied. The objective is to describe the clinicopathological findings in a series of OOCs from a Spanish population that were investigated in relation to the possible presence of HPV. Methods A clinicopathological retrospective analysis followed by a molecular analysis of 28 high- and low-risk HPV genotypes was performed in OOC samples of patients seen during the last 15-years in a Spanish tertiary care center. Results Of 115 odontogenic cysts with keratinization, 16 cases of OOCs were confirmed and evaluated. OOCs occurred predominantly in the mandible of males (mean age…

MaleTertiary careCohort StudiesTertiary Care Centers0302 clinical medicineRecurrenceMedicinePapillomaviridaeBiopsy Needlevirus diseasesMiddle AgedImmunohistochemistryfemale genital diseases and pregnancy complicationsOdontogenicMolecular analysisHPV DNA detectionCell Transformation Neoplastic030220 oncology & carcinogenesisCarcinoma Squamous CellOral and maxillofacial surgeryMouth NeoplasmsAdultmedicine.medical_specialtyHPVAdolescentlcsh:Specialties of internal medicineDents MalaltiesRisk AssessmentYoung Adult03 medical and health scienceslcsh:RC581-951HumansMandibular DiseasesNeoplastic transformationGeneral DentistryRetrospective Studiesbusiness.industryOrthokeratinized odontogenic cystResearch030206 dentistryHigh- and low-riskDermatologyOtorhinolaryngologyOtorhinolaryngologySpainDNA ViralOdontogenic CystsEtiologyNeurology (clinical)HPV High- and low-risk Orthokeratinized odontogenic cyst RecurrencebusinessFollow-Up StudiesHead & Face Medicine
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Rapid and eco-friendly synthesis of graphene oxide-silica nanohybrids

2014

The increasing interest in Graphene oxide (GO) is due to many issues: the presence of both sp2-conjugated atoms and oxygen-containing functional groups provides a strong hydrophilicity and the possibility to further functionalize it with other molecules (i.e. π-π interactions covalent attachment etc.) [1]. Furthermore since the GO is biocompatible and noncytotoxic many studies have been recently focused on the development of GO-based nanodevices for bioimaging DNA detection drug delivery. Due to their low cytotoxicity and large internal surface area silica nanoparticles have been taken into account as promising material for biolabeling and drug loading/delivery. Particular consideration has recently been demonstrated for GO-silica composites because of the potentialities for electrical applications their chemical inertia and stability toward ions exposure. The possibility to combine the extraordinary properties of GO and silica offers several advantages for the realization of nanoprobes for biological applications and of biosensor [12]. The strategy for the fabrication of GO-nanosilica nanohybrids can be schematized as follows: (i) synthesis of GO by oxidizing graphite powder with the method described by Marcano et al. [3] (ii) Preparation of oxygen-loaded silica nanoparticles by thermal treatments in controlled atmosphere in order to induce high NIR emission at 1272 nm from high purity silica nanoparticles. (iii) preparation of GrO-silica nanohybrid films via rapid solvent casting in water. The nanohybrids were tested by XPS FTIR Raman analysis UV photoluminescence analysis TGA Zeta potential measurements electrical tests AFM and SEM. Several nanohybrids were prepared by combining two different typologies of GO and two different samples of silica.
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Dendrimer-encapsulated and cored metal nanoparticles for electrochemical nanobiosensing

2014

Abstract We discuss nanosized dendrimers from the point of view of electrochemical-biosensor design. We review electrochemical biosensors based on dendrimer-encapsulated metal nanoparticles (NPs) and their various applications (e.g., measurement of small biological and toxic molecules, detection of DNA, and immunosensing of disease biomarkers). Finally, we discuss some future applications of electrochemical biosensors based on dendrimer-encapsulated metal NPs.

chemistry.chemical_classificationMaterials scienceBiomoleculeNanotechnologyElectrochemistryAnalytical ChemistryMetalDna detectionchemistryDendrimervisual_artvisual_art.visual_art_mediumElectrochemical biosensorDisease biomarkerMetal nanoparticlesSpectroscopyTrAC Trends in Analytical Chemistry
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